� Combining the specificity of small interfering RNA (siRNA) silencing with the versatility of lentiviral vectors gives researchers a potent tool for the investigation of gene function both in vivo and in vitro. This month's offspring of Cold Spring Harbor Protocols features a match of protocols from Inder Verma and colleagues at the Salk Institute describing this method for achieving long-term down-regulation of specific target genes in a wide range of prison cell types. "Design and Cloning of an shRNA into a Lentiviral Vector" is freely approachable on the website for Cold Spring Harbor Protocols.
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